Decellularized porcine cortical bone fragments were implanted to rat subcutaneously. Rat host cells infiltrated to the gap between assembled fragments 1 week after implantation. Two weeks after implantation, the gap was not discernible on X-ray micro CT images, and a bone-like collagen matrix was observed in the gap histologically. These findings suggest that the assembled decellularized bone fragments induce formation of bone matrix to fill the gap in vivo.
Figure. Evaluation of osteogenesis between decellularized cortical bone fragments. (a, d) X-ray micro CT images. (b, c, e, f) H-E stained sections. (c, f) High magnification of b and e. (a-c) 1 week after implantation, (d-f) 2 weeks after implantation. (g) Schematic illustration for formation of bone-like tissue between decellularized cortical bone fragments.
The dialysis fluid flow and solute removal performance of newly developed dialyzers and a downsized dialyzer were evaluated using a dimensionless correlation equation related to the mass transfer coefficient of the dialysate-side film in a mass transfer model, which was used in quantitative analyses in our previous study.
Melanoma is a malignant tumor occurring typically in the skin.
Good mass transfer and high cell density culture are required for bioreactors using animal cells.
Ventricular activation time (AT [ms]) is the time required to activate the ventricle electrically.
To relieve or manage stress, objective assessment of stress is required.