Decellularized porcine cortical bone fragments were implanted to rat subcutaneously. Rat host cells infiltrated to the gap between assembled fragments 1 week after implantation. Two weeks after implantation, the gap was not discernible on X-ray micro CT images, and a bone-like collagen matrix was observed in the gap histologically. These findings suggest that the assembled decellularized bone fragments induce formation of bone matrix to fill the gap in vivo.
Figure. Evaluation of osteogenesis between decellularized cortical bone fragments. (a, d) X-ray micro CT images. (b, c, e, f) H-E stained sections. (c, f) High magnification of b and e. (a-c) 1 week after implantation, (d-f) 2 weeks after implantation. (g) Schematic illustration for formation of bone-like tissue between decellularized cortical bone fragments.